Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Rapid Assessment of Membrane Protein Quality by…
JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
Journal (Biochemistry)
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Journal (Biochemistry)
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
 

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Article DOI: 10.3791/64322-v • 06:26 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1, 1
1Peak Proteins Ltd.

Chapters

Summary
Automatic Translation

The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

Tags

Fluorescent Size Exclusion Chromatography SEC Profile Membrane Protein Quality FSEC Sample Preparation Solubilization Buffer Detergent Stock Low-speed Centrifugation High-speed Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter