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Uma plataforma de alto rendimento para cultura e imagem 3D de organoides
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Uma plataforma de alto rendimento para cultura e imagem 3D de organoides

Article DOI: 10.3791/64405-v • 07:42 min • October 14th, 2022
October 14th, 2022 •
1,2, 1, 1, 1, 1, 1, 1,3,4, 1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

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Este trabalho apresenta um protocolo de fabricação para um novo tipo de substrato de cultura com centenas de microrecipientes por mm2, no qual organoides podem ser cultivados e observados por microscopia de alta resolução. Os protocolos de semeadura celular e imunocoloração também são detalhados.

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Biologia Edição 188
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