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Journal / Biology / Quantifizierung zirkulärer RNAs mittels digitaler …
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Biology
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JoVE Journal Biology
Quantifizierung zirkulärer RNAs mittels digitaler Tröpfchen-PCR
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Quantifizierung zirkulärer RNAs mittels digitaler Tröpfchen-PCR

Article DOI: 10.3791/64464-v • 08:39 min • September 16th, 2022
September 16th, 2022 •
*1,2, *1,2, 1
1Institute of Life Sciences, 2School of Biotechnology, KIIT University
* These authors contributed equally

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Dieses Protokoll beschreibt die detaillierte Methode der digitalen Tröpfchen-PCR (dd-PCR) zur präzisen Quantifizierung von zirkulären RNA (circRNA)-Spiegeln in Zellen unter Verwendung divergenter Primer.

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Biologie Ausgabe 187 Circular RNA RT-PCR divergente Primer absolute Quantifizierung
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