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Ex Vivo Analisi di transitori di Ca2+ attivati meccanicamente in cellule uroteliali
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Ex Vivo Analisi di transitori di Ca2+ attivati meccanicamente in cellule uroteliali

Article DOI: 10.3791/64532-v • 05:35 min • September 28th, 2022
September 28th, 2022 •
1,2, 1, 1,2
1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh

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Questo protocollo descrive una metodologia per valutare la funzione dei canali ionici attivati meccanicamente nelle cellule uroteliali native utilizzando il sensore fluorescente Ca2+ GCaMP5G.

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Medicina Numero 187 Meccanotrasduzione Ca2+ imaging canali ionici stimolazione meccanica GCaMP5G
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