Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 筛选精子以快速分离斑马鱼的种系编辑…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
筛选精子以快速分离斑马鱼的种系编辑
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Journal (Genetics)
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
Journal (Genetics)
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
 
Click here for the English version

筛选精子以快速分离斑马鱼的种系编辑

Article DOI: 10.3791/64686-v • 05:55 min • February 10th, 2023
February 10th, 2023 •
1, 2, 1,2
1Department of Molecular Biosciences, The University of Texas at Austin, 2Department of Nutritional Sciences, The University of Texas at Austin

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

CRISPR-Cas技术彻底改变了基因组编辑领域。然而，寻找和分离所需的种系编辑仍然是一个主要瓶颈。因此，该协议描述了一种使用标准PCR，限制性酶切和凝胶电泳技术快速筛选F0 CRISPR注射斑马鱼精子以进行种系编辑的稳健方法。

Tags

本月在JoVE上，第192期，CRISPR-CAS9，斑马鱼，精子DNA，基因组编辑
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter