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Journal / Genetics / Screening af sædceller for hurtig isolering af…
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Genetics

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JoVE Journal Genetics
Screening af sædceller for hurtig isolering af kimlinjeredigeringer hos zebrafisk
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Journal (Genetics)
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Journal (Genetics)
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
 
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Screening af sædceller for hurtig isolering af kimlinjeredigeringer hos zebrafisk

Article DOI: 10.3791/64686-v • 05:55 min • February 10th, 2023
February 10th, 2023 •
1, 2, 1,2
1Department of Molecular Biosciences, The University of Texas at Austin, 2Department of Nutritional Sciences, The University of Texas at Austin

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CRISPR-Cas-teknologier har revolutioneret genomredigering. At finde og isolere den ønskede kimlinjeredigering er dog fortsat en stor flaskehals. Derfor beskriver denne protokol en robust metode til hurtig screening af F0 CRISPR-injiceret zebrafisksæd for kimlinjeredigeringer ved hjælp af standard PCR-, restriktionsfordøje- og gelelektroforeseteknikker.

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Denne måned i JoVE udgave 192 CRISPR-CAS9 zebrafisk sæd-DNA genomisk redigering
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