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JoVE Journal Genetics
Ikke-vandig isolering og berigelse af kirtelkapiteller stilkede og sessile trichomer fra Cannabis sativa
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Ikke-vandig isolering og berigelse af kirtelkapiteller stilkede og sessile trichomer fra Cannabis sativa

Article DOI: 10.3791/64798-v • 08:18 min • May 12th, 2023
May 12th, 2023 •
1, 1, 1, 2, 2, 1, 3, 3, 4, 2, 3, 1
1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center

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En protokol præsenteres for bekvem og høj gennemstrømning isolering og berigelse af kirtelcapitate stilkede og sessile trichomer fra Cannabis sativa. Protokollen er baseret på en tør, ikke-bufferekstraktion af trichomer ved kun at bruge flydende nitrogen, tøris og nylonsigter og er velegnet til RNA-ekstraktion og transkriptomisk analyse.

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Genetik nr. 195
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