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Journal / Cancer Research / Anvendelse af AlDeSense til stratificering af…
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JoVE Journal Cancer Research
Anvendelse af AlDeSense til stratificering af kræftceller i æggestokkene baseret på aldehyddehydrogenase 1A1-aktivitet
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Anvendelse af AlDeSense til stratificering af kræftceller i æggestokkene baseret på aldehyddehydrogenase 1A1-aktivitet

Article DOI: 10.3791/64713-v • 09:59 min • March 31st, 2023
March 31st, 2023 •
*1,3,4, *2,3,4, 1,3,4, 1,2,3,4
1Department of Chemistry, University of Illinois Urbana-Champaign, 2Department of Biochemistry, University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 4Cancer Center of Illinois, University of Illinois Urbana-Champaign
* These authors contributed equally

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Metoder til måling af ALDH1A1-aktivitet i levende celler er kritiske i kræftforskning på grund af dets status som en biomarkør for stamme. I denne undersøgelse anvendte vi en isoform-selektiv fluorogen sonde til at bestemme de relative niveauer af ALDH1A1-aktivitet i et panel af fem ovariecancercellelinjer.

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Denne måned i JoVE nummer 193
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