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Journal / Environment / ゼブラフィッシュ胚および肝臓細胞株のスフェロイドの3D培養法…
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JoVE Journal Environment
ゼブラフィッシュ胚および肝臓細胞株のスフェロイドの3D培養法
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ゼブラフィッシュ胚および肝臓細胞株のスフェロイドの3D培養法

Article DOI: 10.3791/64859-v • 08:00 min • January 20th, 2023
January 20th, 2023 •
1, 2, 2, 1, 1, 2, 3, 4, 2, 4
1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Cell Biology, Federal University of Paraná (UFPR), 4Department of Genetics, Federal University of Paraná (UFPR)

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ここでは、2つのゼブラフィッシュ(ダニオレリオ)細胞株、ZEM2S(胚)とZFL(正常肝細胞)のスフェロイドを形成するための効果的で簡単かつ高速な3D培養プロトコルを紹介します。

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