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Journal / Biochemistry / Kısa Biyotinile RNA Kullanan RNA Bağlayıcı…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Kısa Biyotinile RNA Kullanan RNA Bağlayıcı Proteinlerin Optimize Edilmiş Kantitatif Aşağı Çekme Analizi
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Kısa Biyotinile RNA Kullanan RNA Bağlayıcı Proteinlerin Optimize Edilmiş Kantitatif Aşağı Çekme Analizi

Article DOI: 10.3791/64923-v • 07:55 min • February 17th, 2023
February 17th, 2023 •
1, 2, 2, 1,3, 1
1Center for Human Technology, Italian Institute of Technology (IIT), 2Department of Experimental Oncology, European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome

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Burada, insan hücrelerinden toplam protein ekstraktını, biyotinile RNA ile kaplanmış streptavidin boncuklarını ve kütle spektrometri analizini kullanarak, spesifik RNA dizilerinin protein interaktörlerini ortaya çıkarmak, ölçmek ve doğrulamak için optimize edilmiş bir in vitro yöntem sunuyoruz.

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JoVE'de Bu Ay Sayı 192 RNA-protein etkileşimleri protein aşağı çekme RNA bağlayıcı proteinler (RBP'ler) biyotinile RNA RNA aptamerleri
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