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Journal / Biochemistry / Un'analisi pull-down quantitativa ottimizzata…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Un'analisi pull-down quantitativa ottimizzata delle proteine leganti l'RNA utilizzando RNA biotinilato corto
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Un'analisi pull-down quantitativa ottimizzata delle proteine leganti l'RNA utilizzando RNA biotinilato corto

Article DOI: 10.3791/64923-v • 07:55 min • February 17th, 2023
February 17th, 2023 •
1, 2, 2, 1,3, 1
1Center for Human Technology, Italian Institute of Technology (IIT), 2Department of Experimental Oncology, European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome

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Qui, presentiamo un metodo in vitro ottimizzato per scoprire, quantificare e convalidare gli interattori proteici di specifiche sequenze di RNA, utilizzando estratto proteico totale da cellule umane, perle di streptavidina rivestite con RNA biotinilato e analisi di spettrometria di massa.

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Questo mese in JoVE Numero 192 interazioni RNA-proteina pull-down proteico RNA-binding proteins (RBPs) RNA biotinilato RNA aptameri RNA
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