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JoVE Journal
Biochemistry

This content is Open Access.

How to Stabilize Protein
 

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Article doi: 10.3791/58666
February 11th, 2019

Summary February 11th, 2019

A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. Buffer systems, salts and additives, together comprising three unique stability screens, are assayed with proteins to identify suitable buffers for functional and structural studies.

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