Journal
/
Neuroscience
/
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

DOI:
10.3791/64953-v

•

09:39 min

•

December 16, 2022

•

, , , , ,
1School of Neuroscience,Virginia Polytechnic Institute and State University, 2CMU-Pitt Joint Computational Biology, School of Medicine,University of Pittsburgh, 3Eastern Virginia Medical School, 4Department of Physics,University of Miami

Chapters

  • 00:05Introduction
  • 00:33Calcium Imaging
  • 01:38Analysis of 3D Calcium Imaging Data by TACI
  • 07:29Results: Using TACI to Analyze 3D Calcium Imaging
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

TrackMate Analysis of Calcium Imaging (TACI) is an open-source ImageJ plugin for 3D calcium imaging analysis that examines motion on the z-axis and identifies the maximum value of each z-stack to represent a cell's intensity at the corresponding time point. It can separate neurons overlapping in the lateral (x/y) direction but on different z-planes.

Tags

Keywords: 3D Calcium ImagingImageJ PluginTACIMotion CorrectionZ-driftTemperature RecordingPeltierTIFF File RenamingTIFF File Organizing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64953?article_type=v

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
check_url/64953?article_type=v

Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe
check_url/64953?article_type=v

Functional Calcium Imaging in Developing Cortical Networks
check_url/64953?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/64953?article_type=v

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
check_url/64953?article_type=v

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
check_url/64953?article_type=v

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
check_url/64953?article_type=v

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
check_url/64953?article_type=v

Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus (Video) | JoVE
check_url/64953?article_type=v

An Innovative Approach to Neural Electrical Stimulation Using Calcium Imaging (Video) | JoVE

Read Article