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En enkel teknikk for å analysere lokomotorisk aktivitet i Drosophila

Article DOI: 10.3791/65092-v • 07:47 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1,2
1Institute of Neuroscience, Soochow University, 2Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University

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Den nåværende protokollen vurderer den lokomotoriske aktiviteten til Drosophila ved å spore og analysere bevegelsen av fluer i en håndlaget arena ved hjelp av åpen kildekode-programvare Fiji, kompatibel med plugins for å segmentere piksler i hver ramme basert på HD-videoopptak for å beregne parametere for hastighet, avstand, etc.

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Denne måneden i JoVE utgave 192 Drosophila melanogaster lokomotorisk aktivitet AnimalTracker API Fiji nevrodegenerativ sykdom atferdsmangel
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