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Immunology and Infection

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JoVE Journal Immunology and Infection
Arricchimento di vescicole extracellulari native e ricombinanti di micobatteri
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Arricchimento di vescicole extracellulari native e ricombinanti di micobatteri

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Questo protocollo descrive in dettaglio l'arricchimento di vescicole extracellulari micobatteriche native (mEV) da colture axeniche di Mycobacterium smegmatis (Msm) e come possono essere progettate e arricchite le MsmEV ricombinanti contenenti mCherry (un reporter fluorescente rosso). Infine, verifica il nuovo approccio con l'arricchimento di MsmEVs contenenti la proteina EsxA di Mycobacterium tuberculosis.

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Immunologia e infezione Numero 202 Vescicole extracellulari vescicole ricombinanti Esat-6 micobatteri ExsA mCherry Cfp-29 batteri
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