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Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
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1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute,NCR Biotech Science Cluster, 2Clinical Microbiology Division,CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute,Rutgers University
Chapters
- 00:00Introduction
- 00:42Growing Mycobacterium smegmatis
- 02:31Enrichment of Mycobacterium smegmatis EVs by Density Gradient Centrifugation
- 05:24Results: Verifying Enrichment of MsmEVs Containing the EsxA Protein of M. tuberculosis
- 06:19Conclusion
This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.
