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JoVE Journal Cancer Research
Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE
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Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE

In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

Article DOI: 10.3791/65286-v • 02:03 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1,2,3,4,5
1Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, 3Department of Biomedical Sciences, Cedars-Sinai Medical Center, 4Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 5Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles
* These authors contributed equally

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मॉडलिंग ब्रेन ट्यूमर विवो में इलेक्ट्रोपोरेशन-आधारित डिलीवरी प्लास्मिड डीएनए रोगी उत्परिवर्तन हस्ताक्षर ट्यूमर मॉडल प्रीक्लिनिकल परीक्षण इम्यूनोथेरेपी माउस मॉडल ट्यूमर आबादी प्रतिरक्षा कोशिका आबादी उपचार प्रतिक्रिया ऑर्थोटोपिक प्रत्यारोपण स्थापित ट्यूमर सेल लाइनें व्यक्तिगत प्रतिनिधित्व रोगी-विशिष्ट ट्यूमर उत्परिवर्तन डीएनए निर्माण तंत्रिका अग्रदूत कोशिकाएं (एनपीसी) दोहरे-रिकोम्बिनेस-मध्यस्थता कैसेट एक्सचेंज (एमएडीआर) दैहिक म्यूटेनेसिस के साथ मोज़ेक विश्लेषण। ड्राइवर म्यूटेशन नवजात माउस पिल्ले विभाजित कोशिकाएं पार्श्व वेंट्रिकल्स डीएनए प्लास्मिड के माइक्रोइंजेक्शन ट्रांसपोसन सीआरआईएसपीआर-निर्देशित एसजीआरएनए
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