A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Genri Numata; Eiki Takimoto

doi:10.3791/65384

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A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

JoVE Journal
Biology

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JoVE Journal Biology

A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

1,429 Views

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07:49 min

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July 21, 2023

DOI:

10.3791/65384-v

DOI:

10.3791/65384-v

•

07:49 min

July 21, 2023

•

1377 Views

Genri Numata^1,2, Eiki Takimoto^1,3

¹Department of Cardiovascular Medicine,The University of Tokyo Hospital ²Department of Therapeutic Strategy for Heart Failure, Graduate School of Medicine,The University of Tokyo ³Division of Cardiology, Department of Medicine,The Johns Hopkins Medical Institutions

Transcript

The heart rate-related relationship is important for determining cardiac characteristics, particularly, in HFpEF. This protocol aims to assess heart rate-dependent cardiac function. Any experimental models, available modalities for assessing heart rate-related cardiac function, are limited to treadmill exercise testing.

The treadmill test, however, is influenced by variables such as body weight. To overcome these limitations, we have developed around atrial-pacing protocol to detect subtle, yet significant changes in the later performance that are linked to heart rate. Our protocol offers a variable tool for identifying cardiac dysfunction, manifested by heart rate changes.

It also presents a number approach to studying diastolic dysfunction in mirroring heart failure models, particularly in HFpEF.

Summary

The present protocol describes obtaining the pressure-volume relationship through transesophageal pacing, which serves as a valuable tool in evaluating diastolic function in mouse models of heart failure.