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Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli
Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from <em>E. coli</em>
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli

Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli

7,214 Views

05:19 min

June 30, 2023

DOI:

05:19 min
June 30, 2023

7145 Views
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Summary

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The present protocol describes a detailed method for the bacterial production of recombinant proteins, including typically insoluble or disulfide-bond containing proteins, packaged inside extracellular membrane-bound vesicles. This has the potential to be applied to versatile areas of scientific research, including applied biotechnology and medicine.

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