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05:19 min
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June 30, 2023
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Summary
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The present protocol describes a detailed method for the bacterial production of recombinant proteins, including typically insoluble or disulfide-bond containing proteins, packaged inside extracellular membrane-bound vesicles. This has the potential to be applied to versatile areas of scientific research, including applied biotechnology and medicine.
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Cite this Article
Streather, B. R., Baker, K., Eastwood, T. A., Mulvihill, D. P. Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli. J. Vis. Exp. (196), e65442, doi:10.3791/65442 (2023).
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