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Neuronale Kerne Isolation von Human Postmortem Gehirngewebe
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Neuronale Kerne Isolation von Human Postmortem Gehirngewebe

Article DOI: 10.3791/914 • 10:59 min
October 1st, 2008 •
1, 1
1Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School

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October 1st, 2008

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Die zellulären Heterogenität von Hirngewebe stellt eine erhebliche Einschränkung für das Studium der epigenetischen Markierungen in Chromatin, da die meisten Assays einzelne Zelle Auflösung fehlt. Neuronen sind typischerweise mit Glia und andere nicht-neuronalen Zellen vermischt. Wir bieten ein Protokoll zu extrahieren und zu sammeln neuronale Kerne von menschlichen Gehirns.

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