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Journal / Neuroscience / げっ歯類からの神経細胞とヒト以外の霊長類の脳スライスのDiOLISTIC標識…
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Neuroscience

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JoVE Journal Neuroscience
げっ歯類からの神経細胞とヒト以外の霊長類の脳スライスのDiOLISTIC標識
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げっ歯類からの神経細胞とヒト以外の霊長類の脳スライスのDiOLISTIC標識

Article DOI: 10.3791/2081-v • 09:22 min • July 6th, 2010
July 6th, 2010 •
1, 2, 3, 3, 1
1Section on Neuronal Structure, Laboratory for Integrative Neuroscience, NIAAA, NIH, 2Department Physiology and Pharmacology, Wake Forest University Health Sciences, 3Oregon National Primate Research Center, Division of Neuroscience, Oregon Health and Science University

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我々は、齧歯類と異なる年齢のヒト以外の霊長類の脳スライスの神経細胞に、そのようなDIIなどの蛍光色素を、導入する遺伝子銃の使用を示します。この特定のケースでは、我々は、成体マウス（3-6ヶ月齢）と成人cynomologusサル（9-15歳）を使用します。もともと博士リヒトマン（ガンの研究室で記述されたこの技術、

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Joveの神経科学、問題41、遺伝子銃、樹状突起棘、樹状突起の分岐、神経形態、DII、マウスの脳、cynomogusサルの脳、蛍光標識
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