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Immunology and Infection

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JoVE Journal Immunology and Infection
Induktion och Bedömning av klass Switch rekombination i Renad mus B-celler
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Induktion och Bedömning av klass Switch rekombination i Renad mus B-celler

Article DOI: 10.3791/2130-v • 09:50 min • August 13th, 2010
August 13th, 2010 •
1, 1
1Department of Immunology, University of Toronto

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Efter antigen exponering subpopulationer av aktiverade B-celler genomgå en process som kallas klass växla rekombination (CSR) för att producera antikroppar isotyper med distinkta effektorfunktioner. Det protokoll som beskrivs i denna rapport förklarar hur CSR kan induceras och analyseras

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Immunologi 42 Aktivering-inducerad cytidindeaminas B-celler antikroppar klass Switch rekombination Humoralt immunitet spridning lipopolysackarid CFSE
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