Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / An Allele-specific Gene Expression Assay to Test…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
Journal (Biology)
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
Imaging ATG9A, a Multi-Spanning Membrane Protein (Video) | JoVE
Journal (Biology)
Imaging ATG9A, a Multi-Spanning Membrane Protein (Video) | JoVE
 
Click here for the English version

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Article DOI: 10.3791/2279-v • 10:18 min • November 3rd, 2010
November 3rd, 2010 •
1, 1, 1
1Wellcome Trust Centre for Human Genetics, University of Oxford

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Genetic associations often remain unexplained at a functional level. This method aims to assess the effect of phenotype-associated genetic markers on gene expression by analyzing cells heterozygous for transcribed SNPs. The technology allows accurate measurement by MALDI-TOF mass spectrometry to quantify allele-specific primer extension products.

Tags

Allele-specific Gene Expression Assay Genetic Associations Molecular Level Understanding Gene Expression Mechanism DNA Variants Phenotypes Relative Abundance Of Transcripts Risk Variants Heterozygous Cells DNA Markers Allele Frequency Haplotypic Relationship Cell Types Nucleic Acid Extraction Fibroblasts Primary Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter