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Profiling única célula transcricional dos cardiomiócitos de rato adulto
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Profiling única célula transcricional dos cardiomiócitos de rato adulto

Article DOI: 10.3791/3302-v • 08:24 min • December 28th, 2011
December 28th, 2011 •
1, 2, 1
1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics, University of Washington

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Perfil de expressão única célula permite a análise detalhada de expressão gênica de células individuais. Descrevemos métodos para o isolamento dos cardiomiócitos, e preparar a lisados ​​resultando tanto para microarray transcriptoma todo ou qPCR de metas específicas.

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Biologia Molecular a análise única célula Microarray expressão gênica Cardiomyocyte perfusão do coração do rato ratos qPCR
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