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Journal / Chemistry / Overfladepassivering for Single-molekyle Protein…
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Overfladepassivering for Single-molekyle Protein Studies
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Overfladepassivering for Single-molekyle Protein Studies

Article DOI: 10.3791/50549-v • 10:35 min • April 24th, 2014
April 24th, 2014 •
1, 1, 2, 2, 2, 1
1Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2Department of Chemistry, Seoul National University

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Vi beskriver en fremgangsmåde til passivering af en glasoverflade under anvendelse af polyethylenglycol (PEG). Denne protokol dækker overflade rengøring, overflade funktionalisering og PEG-belægning. Vi introducerer en ny strategi for at behandle overfladen med PEG-molekyler over to runder, som giver overlegen kvalitet af passivering i forhold til eksisterende metoder.

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Kemi single-molekyle spektroskopi polymer polyethylenglycol (PEG) Piranha ætsning amino-silanisering overfladepassivering fluorescens glas overfladebelægning.
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