مثلي الأولية دبقي Xenografts ألخص إختراقي النمو والإيزوسيترات هيدروجيناز أنا الطفرة

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Valadez, J. G., Sarangi, A., Lundberg, C. J., Cooper, M. K. Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation. J. Vis. Exp. (83), e50865, doi:10.3791/50865 (2014).

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Abstract

Materials

Name Company Catalog Number Comments
Phosphate buffered saline Life Technologies 14040-133
Papain dissociation system Worthington Biochemical Corp. LK003150
Trypan blue solution 0.4% Life Technologies 15250061
Ketamine HCl Obtained from institutional pharmacy or local veterinary supply company
Xylazine HCl
Ketoprofen

Ophthalmic ointment

Povidone-iodine

Fisher Scientific

190061617

Cryopreservation medium and proliferation supplement

StemCell Technologies

05751

0.2% Heparin sodium salt in PBS

StemCell Technologies

07980

Penicillin-streptomycin

Life Technologies

15140-122

Dimethyl sulfoxide

Sigma-Aldrich

D6250-5X10ML

NOD.Cg-Prkdcscid I/2rgtm1Wjl/SzJ mice

The Jackson Laboratory

005557

NSG mice

Anti-human vimentin antibody

Dako

M7020

Use 1:200 to 1:800

Anti-human IDH1 R132H antibody

Dianova

DIA-H09

Use 1:100 to 1:400

Centrifuge with swinging bucket rotor

Pipetter with dispensing speed control

Disposable hemocytometer

Fisher Scientific

22-600-100

Sterile surgical gloves

Fisher Scientific

11-388128

Disposable gown

Fisher Scientific

18-567

Surgical mask

Fisher Scientific

19-120-1256

Tuberculin syringe

BD

305620

Alcohol pads

Fisher Scientific

22-246-073

Portable electronic scale

Fisher Scientific

01-919-33

Zoom stereomicroscope

Surgical clipper

Stoelting

51465

Scalpel handle

Fine Science Tools

10003-12

Scalpel blades, #10

Stereotaxic instrument

Stoelting

51730

High-speed drill

Stoelting

51449

Drill bit, 0.6 mm Stoelting 514552

Hamilton syringe

Hamilton

80336

Autoclip, 9 mm

BD

427630

Circulating water warming pad

Kent Scientific

TP-700

TP-1215EA

Hot bead dry sterilizer

Kent Scientific

INS300850

Surgical scissors

Fine Science Tools

14101-14

Fine scissors

Fine Science Tools

14094-11

Spring scissors

Fine Science Tools

15018-10

Dumont forceps

Fine Science Tools

11251-30

Semimicro spatulas

Fisher Scientific

14374

Mouse brain slicer matrix

Zivic Instruments

BSMAS002-1

Cryogenic storage vials

Fisher Scientific

12-567-501

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References

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