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Ein-Kanal-Cell-Attached-Patch-Clamp-Aufnahme

Article DOI: 10.3791/51629-v • 13:07 min • June 9th, 2014
June 9th, 2014 •
1, 2, 1, 3, 4
1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY

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Beschrieben wird hier ein Verfahren zur Erlangung weite Strecken der aktuellen Aufnahme von einem Ionenkanal mit der Cell-Attached-Patch-Clamp-Technik. Diese Methode erlaubt die Beobachtung in Echtzeit, das Muster der Öffnungs-Schließ-Kanal-Konformationen, die die biologische Signal zugrunde liegen. Diese Daten informieren über Kanaleigenschaften in ungestörten biologischen Membranen.

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Neuroscience Ausgabe 88 Biophysik Ionenkanäle Einzel-Kanal-Aufnahme NMDA-Rezeptoren Gating Elektrophysiologie Patch-Clamp kinetische Analyse
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