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Nanogold Labeling do Sistema de levedura Endossomal de Análises ultraestruturais
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Nanogold Labeling do Sistema de levedura Endossomal de Análises ultraestruturais

Article DOI: 10.3791/51752-v • 09:49 min • July 14th, 2014
July 14th, 2014 •
1, 1, 1
1Department of Cell Biology, University Medical Center Utrecht

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A levedura Saccharomyces cerevisiae, tem sido um organismo chave modelo para identificar e estudar genes que regulam a biogênese e funções do sistema endosomal. Aqui apresentamos um protocolo detalhado para a rotulagem específica dos compartimentos endossomais para estudos ultra-estruturais.

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Biologia Celular Edição 89 nanogold carregada positivamente o aumento prata procedimento Tokuyasu microscopia eletrônica rotulagem immunogold fermento
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