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JoVE Journal Neuroscience
Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse
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Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

Article DOI: 10.3791/53303-v • 06:00 min • January 18th, 2016
January 18th, 2016 •
1, 1
1TARC (Translational Animal Research Center), University Medical Center, JGU Mainz

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Summary
Automatic Translation

This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.

Tags

Cortex Hippocampus Thalamus Hypothalamus Lateral Septal Nucleus Striatum In Utero Electroporation C57BL/6 Mouse Cell Manipulation Central Nervous System Signaling Cascades Cell Differentiation Genetically Modified Mice Electrode Position Electroporation Abdominal Cavity Anesthetized Pregnant Mouse Uterine Horns
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