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JoVE Journal Developmental Biology
Isolatie en cultuur van de Adult zebravis Brain-afgeleide Neurosferen
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Isolatie en cultuur van de Adult zebravis Brain-afgeleide Neurosferen

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

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Hier hebben wij een reproduceerbare werkwijze voor volwassenen neurogenese onderzocht onder toepassing van een assay neurosphere afgeleid van de gehele hersenen of van ofwel de telencephalic, tectal of cerebellaire gebieden van de volwassen hersenen zebravis. Daarnaast beschrijven we de procedure om genexpressie te manipuleren in zebravis neurosferen.

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Developmental Biology zebravis neurogenese neurale volwassen stamcellen / voorlopercellen neurosphere assay miRNA genetische manipulatie
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