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Journal / Bioengineering / Die Verbesserung der 2D- und 3D-Haut
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JoVE Journal Bioengineering
Die Verbesserung der 2D- und 3D-Haut
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Die Verbesserung der 2D- und 3D-Haut

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Wir stellen eine Protokoll-Zellen abgeleiteten Matrices reich an extrazellulären Matrixproteinen zu erhalten, unter Verwendung von hochmolekularen Crowders (MMC). Darüber hinaus präsentieren wir ein Protokoll, das MMC in 3D organotypischen Haut Co-Kultur-Generation enthält, die Kulturzeit reduziert, während Laufzeit von Konstrukt beibehalten wird.

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Bioengineering Heft 114 Keratinozyten Fibroblasten makromolekulare crowding 2D-Zellkultur 3D-Zellkultur Haut organotypischen Co-Kulturen extrazelluläre Matrix Kollagen Typ I Kollagen Typ IV Kollagen Typ VII, dermoepidermale Kreuzung
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