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Co-lokalisering af Cell Lineage Markers og tomat Signal

Article DOI: 10.3791/54982-v • 10:56 min • December 28th, 2016
December 28th, 2016 •
1, 1, 1, 1
1Department of Biomedical Sciences, Texas A&M University College of Dentistry

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Vi udviklede to sæt sporing kombinationer i Rosa26 tdtomato (ubikvitært udtrykt i alle celler) / Cre (specifikt udtrykkes i chondrocytter) mus: en med 2.3Col1a1-GFP (specifik for osteoblaster) og en med immunofluorescens (specifikt for knogleceller). Dataene viser den direkte omdannelse af chondrocytter i knogleceller.

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Developmental Biology celle afstamning opsporing celle transdifferentiering immunfluorescens chondrocyt osteoblast osteocyt
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