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JoVE Journal Developmental Biology
Co-localisatie van Cell Lineage Markers en de tomaat Signal
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Co-localisatie van Cell Lineage Markers en de tomaat Signal

Article DOI: 10.3791/54982-v • 10:56 min • December 28th, 2016
December 28th, 2016 •
1, 1, 1, 1
1Department of Biomedical Sciences, Texas A&M University College of Dentistry

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Wij ontwikkelden twee traceren combinaties Rosa26 tdtomato (alomtegenwoordig tot expressie in alle cellen) / Cre (specifiek tot expressie in chondrocyten) muizen: één met 2.3Col1a1-GFP (specifiek voor osteoblasten) en een met immunofluorescentie (specifiek voor botcellen). De gegevens tonen de directe omzetting van chondrocyten in botcellen.

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Developmental Biology cellijn tracing cel transdifferentiatie immunofluorescentie chondrocyten osteoblasten osteocyt
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