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Journal / Genetics / Generering av Fluorescent Protein Fusions i
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JoVE Journal Genetics
Generering av Fluorescent Protein Fusions i
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Generering av Fluorescent Protein Fusions i

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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PCR-mediert gen modifikasjon kan brukes til å generere fluorescerende proteinfusjoner i Candida art, som muliggjør visualisering og kvantifisering av gjærceller og proteiner. Heri presenterer vi en strategi for å konstruere et fluorescerende protein fusjon (Eno1-FP) i Candida parapsilosis.

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Genetikk , PCR-mediert genmodifisering fluoriserende protein, Lithium acetate transformasjon,
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