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Journal / Biochemistry / 공동 침강에 의한 F-actin에 대한 단백질 결합…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
공동 침강에 의한 F-actin에 대한 단백질 결합 측정
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
Journal (Biochemistry)
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Journal (Biochemistry)
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
 
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공동 침강에 의한 F-actin에 대한 단백질 결합 측정

Article DOI: 10.3791/55613-v • 06:17 min • May 18th, 2017
May 18th, 2017 •
1, 2, 1
1Department of Cell Biology, University of Pittsburgh School of Medicine, 2Department of Biology, University of North Carolina at Chapel Hill

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이 프로토콜은 필라멘트 액틴 (F - 굴지)과 함께 공동 침강하는 단백질의 능력을 테스트하는 방법을 설명하고, 바인딩이 관찰되면 상호 작용의 친화력을 측정하는 방법을 설명합니다.

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생화학 액틴 사상 액틴 액틴 - 액틴 액틴 결합 단백질 공 침강 펠렛팅 분석 결합 곡선
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