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Journal / Developmental Biology / Generering av genetiskt modifierade möss genom…
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JoVE Journal Developmental Biology
Generering av genetiskt modifierade möss genom mikroinjektion av oocyter
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Generering av genetiskt modifierade möss genom mikroinjektion av oocyter

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
1, 1
1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

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Mikroinjektionen av musoocyter används vanligtvis för både klassisk transgenes ( dvs den slumpmässiga integrationen av transgener) och CRISPR-medierad geninriktning. Detta protokoll granskar den senaste utvecklingen inom mikroinjektion, med särskild tonvikt på kvalitetskontroll och genotypstrategier.

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Utvecklingsbiologi utgåva 124 möss mikroinjektion pronukleär cytoplasmatisk CRISPR transgen genomredigering oocyter
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