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JoVE Journal Genetics
CRISPR/Cas9-based 跳跃的原始生殖细胞移植在爪
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CRISPR/Cas9-based 跳跃的原始生殖细胞移植在爪

Article DOI: 10.3791/56035-v • 05:34 min • February 1st, 2018
February 1st, 2018 •
1
1Department of Developmental and Cell Biology, University of California, Irvine

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生存所必需的基因构成了创造突变株系的技术障碍。通过将基因组编辑与原始生殖细胞移植结合在一起, 创造出具有系突变的野生型动物, 从而绕过致命性。跳跃也允许在 F1生成中高效生成纯合 null 突变体。在这里, 移植步骤被证明。

Tags

遗传学 问题 132,爪 遗传学 突变体 CRISPR/Cas9 塔伦 基因组编辑 诱变 功能丧失
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