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Journal / Genetics / 使用腺相关病毒 （AAV） 和 2 细胞胚胎电穿孔对大鼠胚胎进行 CRISPR-Cas9…
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Genetics
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JoVE Journal Genetics
使用腺相关病毒 （AAV） 和 2 细胞胚胎电穿孔对大鼠胚胎进行 CRISPR-Cas9 基因组编辑
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使用腺相关病毒 （AAV） 和 2 细胞胚胎电穿孔对大鼠胚胎进行 CRISPR-Cas9 基因组编辑

Article DOI: 10.3791/66069-v • 08:24 min • March 15th, 2024
March 15th, 2024 •
1,2,4, 2, 3, 3, 3, 1,2,3,4
1Animal Modeling Core, University of Missouri, 2Comparative Medicine Program, University of Missouri, 3Rat Resource and Research Center, University of Missouri, 4Department of Veterinary Pathobiology, University of Missouri

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该协议提出了一种用于生产转基因大鼠模型的优化方法。腺相关病毒 （AAV） 用于递送 DNA 修复模板，电穿孔用于递送 CRISPR-Cas9 试剂以完成 2 细胞胚胎的基因组编辑过程。

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本月在JoVE上，第205期，腺相关病毒（AAV），基因组编辑，CRISPR-Cas，电穿孔，2细胞胚胎，敲入，大鼠动物模型
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