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Biochemistry

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JoVE Journal Biochemistry
Caractérisation biochimique et structurelle de l’hydrate de carbone Transport substrat-GRB2 SP0092
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Caractérisation biochimique et structurelle de l’hydrate de carbone Transport substrat-GRB2 SP0092

Article DOI: 10.3791/56294-v • 08:53 min • October 2nd, 2017
October 2nd, 2017 •
1,2, 1,2, 1, 1,2
1Diamond Light Source, Harwell Science & Innovation Campus, 2Research Complex at Harwell, Harwell Science & Innovation Campus

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Un protocole simplifié permettant d’effectuer une caractérisation biochimique et structurelle vaste une protéine de liaison du substrat glucidique de Streptococcus pneumoniae est présenté.

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Transporteurs de biochimie numéro 128 ABC Streptococcus pneumoniae absorption de glucides protéine liant le substrat analyse de décalage thermique multi angle diffusion de la lumière (MALS) chromatographie d’exclusion (SEC) cristallographie rayons x diffraction collecte des données solution de structure de protéine
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