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Journal / Biochemistry / Vaskemiddel-fri ultrahurtig rekonstituering af…
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Biochemistry

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JoVE Journal Biochemistry
Vaskemiddel-fri ultrahurtig rekonstituering af membranproteiner i Lipid dobbeltlag ved hjælp af Fusogenic komplementære-ladet Proteoliposomes.
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Vaskemiddel-fri ultrahurtig rekonstituering af membranproteiner i Lipid dobbeltlag ved hjælp af Fusogenic komplementære-ladet Proteoliposomes.

Article DOI: 10.3791/56909-v • 11:10 min • April 5th, 2018
April 5th, 2018 •
1, 2, 3, 2, 2
1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics, Oxford University, 3Department of Biological Sciences, Southern Methodist University

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Her præsenterer vi to ultrahurtig protokoller til rekonstitution af membranproteiner i fusogenic proteoliposomes og fusion af sådanne proteoliposomes med target lipid dobbeltlag for vaskemiddel-gratis levering af disse Membranproteiner i den postfusion tolagede. Kombinationen af disse tilgange muliggør hurtig og let kontrolleret forsamling af komplekse multi-komponent membran systemer.

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Biokemi spørgsmål 134 Membranproteiner rekonstitution proteoliposomes LUV GUV membran fusion elektron transportkæde ATPsyntase ATP produktion syntetisk biologi
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