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JoVE Journal Biochemistry
Fractionering voor oplossing van oplosbare en onoplosbare Huntingtin soorten
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Fractionering voor oplossing van oplosbare en onoplosbare Huntingtin soorten

Article DOI: 10.3791/57082-v • 07:08 min • February 27th, 2018
February 27th, 2018 •
1,2, 2,3, 4, 1,2,3
1Department of Psychiatry and Human Behavior, University of California Irvine, 2UCI MIND, University of California Irvine, 3Department of Neurobiology and Behavior, University of California Irvine, 4Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center

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Een methode is beschreven voor fractionering van onoplosbare en oplosbare mutant huntingtin soorten van muis hersenen en cel cultuur. De beschreven methode is nuttig voor karakterisering en kwantificering van het huntingtin eiwit flux en aids in het analyseren van eiwitten homeostase in de pathogenese van de ziekte en in het bijzijn van verstoringen

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Biochemie kwestie 132 de ziekte van Huntington aggregatie huntingtin eiwit fractionering onoplosbare proteostasis
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