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JoVE Journal
Immunology and Infection

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人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导
 
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人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导

Article DOI: 10.3791/57804-v 10:28 min July 17th, 2018
July 17th, 2018

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Summary

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脂肪组织血管祖细胞中白、米色脂肪体的分化具有改善肥胖代谢的潜力。我们描述了 CD34+CD31+ 内皮细胞与人的脂肪分离的协议, 并为随后的体外扩增和分化为白色和米色脂肪。讨论了几种下游应用。

Tags

免疫学和感染 问题 137 微血管 CD34+CD31+ 内皮细胞 脂肪组织 成脂 CD34 CD31 胰岛素 罗格列酮 白脂肪细胞 米色脂肪体
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