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JoVE Journal
Biochemistry

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基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物
 
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基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物

Article DOI: 10.3791/58223-v 09:45 min August 18th, 2018
August 18th, 2018

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Summary

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Click here for the English version.

本文介绍了一种用于恢复内在形成的 mRNA 蛋白复合物的串联 RNA 分离过程 (跳闸)。具体来说, rna 蛋白复合物在体内交联, polyadenylated rna 与寡核苷酸 (dT) 珠提取物分离, 特殊基因用修饰 RNA 反义寡核苷酸捕获。通过应用免疫印迹分析, 检测到基因的蛋白质。

Tags

生物化学 问题 138 RNA 结合蛋白 转录后基因调控 polyadenylated RNA ribonucleoprotein 复合体 反义寡核苷酸 酵母,秀丽线虫 人体细胞
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