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JoVE Journal Developmental Biology
Análise de ciclo celular no Germline de c. elegans com o EdU analógico de timidina
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Análise de ciclo celular no Germline de c. elegans com o EdU analógico de timidina

Article DOI: 10.3791/58339-v • 06:58 min • October 22nd, 2018
October 22nd, 2018 •
1,2, 2, 1, 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

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É descrito um método baseado em imagens que pode ser usado para identificar a fase S e analisar a dinâmica do ciclo celular no germline hermafrodita de c. elegans , usando a timidina EdU analógico. Este método não requer nenhum transgenes e é compatível com coloração imunofluorescente.

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Biologia do desenvolvimento questão 140 c. elegans EdU fase S ciclo celular Germline S-fase meiótica M-fase fase G2
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