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Journal / Genetics / Reverse Transkription-Loop-vermittelten…
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JoVE Journal Genetics
Reverse Transkription-Loop-vermittelten Isothermen Verstärkung (RT-Lampe) Assay für Zika-Virus und Housekeeping-Gene in Urin, Serum und Moskito-Proben
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Reverse Transkription-Loop-vermittelten Isothermen Verstärkung (RT-Lampe) Assay für Zika-Virus und Housekeeping-Gene in Urin, Serum und Moskito-Proben

Article DOI: 10.3791/58436-v • 08:03 min • September 14th, 2018
September 14th, 2018 •
1, 2, 2, 1,3, 1,3
1Department of Urology, Beaumont Health System, 2Foundational Sciences, College of Medicine, Central Michigan University, 3Oakland University William Beaumont School of Medicine

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Dieses Protokoll bietet eine effiziente und kostengünstige Methode zum Zika Virus erkennen oder steuern Ziele im menschlichen Urin und Serum-Proben oder Mücken durch reverse Transkription Schleife-vermittelten Isothermen Amplifikation (RT-Lampe). Diese Methode erfordert keine RNA isoliert und kann innerhalb von 30 Minuten erfolgen.

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Genetik Ausgabe 139 Zika-Virus RT-Lampe Urin Serum Aedes Point-of-Care testing Feld-Tools
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