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Journal / Cancer Research / Echtzeit-Erkennung von In Vitro Tumor Zelle…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Echtzeit-Erkennung von In Vitro Tumor Zelle Apoptose induziert durch CD8+ T-Zellen, unterdrückende Immunfunktionen Tumor infiltrieren myeloische Zellen zu studieren
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Echtzeit-Erkennung von In Vitro Tumor Zelle Apoptose induziert durch CD8+ T-Zellen, unterdrückende Immunfunktionen Tumor infiltrieren myeloische Zellen zu studieren

Article DOI: 10.3791/58841-v • 09:57 min • January 29th, 2019
January 29th, 2019 •
*1,2, *3, 2, 3,4
1Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh, 3Edinburgh Phenotypic Assay Centre, University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine, University of Edinburgh
* These authors contributed equally

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Wir beschreiben hier ein Protokoll zur Zytotoxizität von pre-activated CD8 untersuchen+ T-Zellen gegen Krebszellen durch das Erkennen von apoptotischen Krebs Zellen über Echtzeit-Mikroskopie. Dieses Protokoll kann Mechanismen hinter myeloische Zellen-induzierte T-Zell-Unterdrückung untersuchen und zu bewerten Verbindungen auffüllen T Zellen durch Blockade von Immunzellen suppressive myeloische abzielen.

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Cancer Research Ausgabe 143 Apoptose Krebszelle CD8+ T Zelle Tumor-assoziierten Makrophagen Zytotoxizität Fluoreszenz-Mikroskopie myeloische abgeleitet Suppressor-Zellen
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