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Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

DOI:
10.3791/58841-v

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09:57 min

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January 29, 2019

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, , ,
1Royal (Dick) School of Veterinary Studies and Roslin Institute,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh, 3Edinburgh Phenotypic Assay Centre,University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine,University of Edinburgh

Chapters

  • 00:04Title
  • 00:59Isolated CD8+ T Cell Activation and Expansion
  • 01:49Pre-Activated CD8+ T Cell:Target Cell Co-Culture
  • 04:49Cell Imaging and Analysis
  • 07:39Results: Representative Effector CD8+ T Cell and Target Cancer Cell Interactions
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a protocol to investigate cytotoxicity of pre-activated CD8+ T cells against cancer cells by detecting apoptotic cancer cells via real-time microscopy. This protocol can investigate mechanisms behind myeloid cell-induced T cell suppression and evaluate compounds aimed at replenishing T cells via blockade of immune suppressive myeloid cells.

Tags

Keywords: Tumor Cell ApoptosisCD8+ T CellsTumor-infiltrating Myeloid CellsImmune SuppressionCancer ImmunologyAutoimmune DiseasesCell-to-cell InteractionsCheckpoint InhibitorCell CultureTarget CellsT-cell Activation

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