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JoVE Journal Biochemistry
Fracionamento celular de células U937, na ausência de centrifugação de alta velocidade
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Fracionamento celular de células U937, na ausência de centrifugação de alta velocidade

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Aqui, apresentamos um protocolo para isolar a membrana plasmática, citoplasma e mitocôndrias de células U937 sem o uso de centrifugação de alta velocidade. Esta técnica pode ser usada para purificar as fracções subcelulares para posterior exame de localização de proteínas através de immunoblotting.

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Bioquímica edição 143 fracionamento celular mitocôndria membrana citoplasma organelas centrifugação U937
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