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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation og adoptiv overførsel af højt saltindhold behandlet Antigen-præsenterer dendritiske celler
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Isolation og adoptiv overførsel af højt saltindhold behandlet Antigen-præsenterer dendritiske celler

Article DOI: 10.3791/59124-v • 09:29 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1, 1, 1
1Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center

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Vi præsenterer her, en protokol for at isolere dendritiske celler fra murine milt af magnetiske celle sortering og efterfølgende adoptiv overførsel til naive mus. Model af høj-salt aktiveret dendritiske celler blev valgt til at forklare de trinvise procedurer adoptiv overførsel og flowcytometri.

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Immunologi og infektion sag 145 betændelse adoptiv overførsel dendritiske celle Isolation dendritiske celler forhøjet blodtryk immun celle sortering flowcytometri
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