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Journal / Developmental Biology / rRNA 2'-O-メチル化のDNAzyme依存性分析
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Developmental Biology

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JoVE Journal Developmental Biology
rRNA 2'-O-メチル化のDNAzyme依存性分析
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rRNA 2'-O-メチル化のDNAzyme依存性分析

Article DOI: 10.3791/59700-v • 09:12 min • September 16th, 2019
September 16th, 2019 •
1, 1
1School of Biosciences, University of Birmingham

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ここでは、RNAのDNAzyme依存性切断に関するプロトコルを提示する。これにより、RNA 2'-O-メチル化の迅速かつ部位依存性の分析が可能になります。このアプローチは、snoRNA活性の予備的または主要な評価に使用することができる。

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発生生物学 問題 151 RNA 修飾 2'-O-メチル化 rRNA DNAzyme snoRNA RNA分析
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